2×TransStart? FastPfu PCR SuperMix (+dye)
2×TransStart FastPfu PCR 預混液(+dye)
| 目錄號 | 規格 | 單價(jià) |
|---|---|---|
| AS221-11 | 1 ml | 550 |
| AS221-12 | 5×1 ml | 2340 |
產(chǎn)品詳情介紹
本產(chǎn)品包含TransStart? FastPfu DNA Polymerase、dNTPs和優(yōu)化的反應緩沖液，濃度為2×，擴增效率強，擴增速度快，具有高保真性、高特異性。DNA擴增時(shí)，只需加入模板、引物和水，使SuperMix溶液的濃度為1×即可進(jìn)行反應。擴增產(chǎn)物為平端，可直接克隆于pEASY?-Blunt系列載體中。2×TransStart? FastPfu PCRSuperMix (+dye)擴增產(chǎn)物可直接點(diǎn)樣電泳，如用于克隆，需純化去掉染料。其PCR產(chǎn)物不適用于聚丙烯酰胺凝膠電泳。
? 減少PCR擴增操作時(shí)間。
? 避免因多步操作帶來(lái)的污染。
? 保真性是EasyTaq? DNA Polymerase的54倍。
? 基因組DNA片段的擴增(≤15 kb)。
? Plasmid DNA片段擴增(≤20 kb)。
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3 Chen K, Hu Z, Song W, et al. Diversity of O-glycosyltransferases contributes to the biosynthesis of flavonoid and triterpenoid glycosides in Glycyrrhiza uralensis[J]. ACS Synthetic Biology, 2019.(IF 5.57)
4 Wang Y, Wang Z, Chen Y, et al. A highly efficient CRISPR-Cas9-based genome engineering platform in Acinetobacter baumannii to understand the H2O2-sensing mechanism of OxyR[J]. Cell Chemical Biology, 2019.(IF 6.76)
