TransScript? One-Step gDNA Removal and cDNA Synthesis SuperMix
TransScript一步法去除gDNA及第一鏈cDNA合成試劑盒
目錄號： AT311-02
單 價(jià)：￥1160
產(chǎn)品詳情介紹
本產(chǎn)品以RNA為模板，在同一反應體系中，合成第一鏈cDNA的同時(shí)去除RNA模板中殘留的基因組DNA。反應結束后，只需在85℃加熱5秒鐘，即可同時(shí)失活TranScript? RT/RI與gDNA Remover。
? 在同一反應體系中，同時(shí)完成反轉錄與基因組DNA的去除，操作簡(jiǎn)便，降低污染機率。
? 產(chǎn)物用于qPCR：反轉錄15分鐘；產(chǎn)物用于PCR：反轉錄30分鐘。
? 反應結束后，同時(shí)熱失活RT/RI與gDNA Remover。與傳統的用DNase I預處理RNA的方法相比，避免了處理后熱失活DNase I對RNA的損傷。
? 操作簡(jiǎn)單。
?合成片段≤12 kb。
使用前請將各組分點(diǎn)甩離心
產(chǎn)品穩定性
使用不同批次產(chǎn)品分別以人總RNA為模板，進(jìn)行RT-PCR檢測，1.0%瓊脂糖凝膠電泳分析反轉錄效果。
使用不同批次產(chǎn)品分別以人100 ng總RNA、人100 ng總RNA+200 ng gDNA、200 ng gDNA為模板，進(jìn)行RT-PCR檢測，1.0%瓊脂糖凝膠電泳分析模板DNA去除效果；qRT-PCR檢測18S RNA表達量。
與競品的比較
使用TransGen、Company TA和Company TH產(chǎn)品，分別以人100 ng總RNA、人100 ng總RNA+200 ng gDNA、200 ng gDNA為模板，進(jìn)行RT-PCR檢測， 1.0%瓊脂糖凝膠電泳分析模板DNA去除效果；qRT-PCR檢測18S RNA表達量。
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